|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31927||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3999
Modifications to backboneSeveral amino acids between His-tag and rsTagRFP were removed.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Alt namereversibly photoswitchable TagRFP red fluorescent protein
Insert Size (bp)702
MutationF84W/I125L/N148A/S165G/F181L/K189N/Y200F compared to TagRFP (but numbering relative to EGFP)
- Promoter araB
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBAD-fwd
- 3′ sequencing primer pBAD-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD/HisD-rsTagRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31927 ; http://n2t.net/addgene:31927 ; RRID:Addgene_31927)
For your References section:Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. Subach FV, Zhang L, Gadella TW, Gurskaya NG, Lukyanov KA, Verkhusha VV. Chem Biol. 2010 Jul 30;17(7):745-55. 10.1016/j.chembiol.2010.05.022 PubMed 20659687