|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31945||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3999
Modifications to backboneSeveral amino acids between His-tag and PATagRFP were removed.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Alt namephotoactivatable dark-to-red TagRFP red fluorescent protein
Insert Size (bp)702
MutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H203R/R207I/V218W/C229S compared to TagRFP (but numbering relative to EGFP)
- Promoter araB
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBAD-fwd
- 3′ sequencing primer pBAD-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD/HisD-PATagRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31945 ; http://n2t.net/addgene:31945 ; RRID:Addgene_31945)
For your References section:Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV. J Am Chem Soc. 2010 May 12;132(18):6481-91. 10.1021/ja100906g PubMed 20394363