Addgene - pEGFP-STAG2-S653Stop Plasmid Data

Plasmid 31976: pEGFP-STAG2-S653Stop

Gene/insert name:
human STAG2 cDNA
Alt name:
stromal antigen 2
Alt name:
SA-2
Species:
H. sapiens (human)
Entrez Gene:
STAG2 (RP11-517O1.1, SA-2, SA2, SCC3B, bA517O1.1)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Mutation:
changed Serine 653 to Stop codon
Vector backbone:
pEGFP-C3
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4727
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
gcatttggatgccttattgc List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Todd Waldman
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mutational inactivation of STAG2 causes aneuploidy in human cancer. Solomon et al (Science. 2011 Aug 19;333(6045):1039-43. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31976" in your Materials and Methods section.