Addgene - mCherry-SEpHluorin Plasmid Data

Plasmid 32001: mCherry-SEpHluorin

Gene/insert name:
mCherry
Insert size:
902
Fusion protein or tag:
Superecliptic pHluorin
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Modifications to Backbone:
The EGFP coding region from the EGFP-N1 vector was replaced with a PCR product containing the SEpHluorin coding region flanked by BamHI and NotI restriction sites.
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
5'd[CGTCGCCGTCCAGCTCGACCAG]3' List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Principal Investigator:
Sergio Grinstein
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. Koivusalo et al (J Cell Biol. 2010 Feb 22;188(4):547-63. Epub 2010 Feb 15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32001" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

32002: Lyn-tailed mCherry-SEpHluorin