Addgene - pMN437 Plasmid Data

Plasmid 32362: pMN437

Gene/insert name:
gfpm2+
Alt name:
codon optimized GFP for mycobacteria
Vector backbone:
pMS2
(Search Vector Database)
Vector type:
Mycobacterial GFP expression vector
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
psmyc1 List of Sequencing Primers
Bacterial resistance(s)
Hygromycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Michael Niederweis
Terms and Licenses:
MTA

Comments:

This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).

Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of outer membrane proteins of Mycobacterium tuberculosis. Song et al (Tuberculosis (Edinb). 2008 Nov;88(6):526-44. Epub 2008 Apr 25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32362" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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