Addgene - punc-119cbr Plasmid Data

Plasmid 32568: punc-119cbr

Gene/insert name:
unc-119
Insert size:
2155
Species:
C. briggsae
GenBank ID:
U45326.1
Vector backbone:
punc-119c
(Search Vector Database)
Vector type:
Worm Expression
Backbone size w/o insert (bp):
1413
Cloning site 5':
PstI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
GTAACATCAGAGATTTTGAGACAC List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
Pir1
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
unc-119 (C. briggsae)
Person or lab that originally
cloned the gene/insert:
C. briggsae unc-119 gene was PCR amplified from pCFJ151.
Sequence: View sequences (2)
Principal Investigator:
Al Fisher
Terms and Licenses:
MTA

Comments:

Plasmid is similar to punc-119c but has C. briggsae unc-119 genomic DNA in place of C. elegans unc-119 promoter and cDNA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32568" in your Materials and Methods section.