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pPK605
(Plasmid #38148)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38148 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR)
  • Alt name
    unc-119
  • Species
    C. elegans (nematode)
  • Entrez Gene
    unc-119 (a.k.a. CELE_M142.1)
  • Promoter unc-119

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
  • Species
    C. elegans (nematode)
  • Promoter pie-1 promoter

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13pUC-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was derived from pID3.01B (Geraldine Seydoux - Addgene plasmid 22720)
  • Terms and Licenses

Depositor Comments

Plasmid pPK605 contains two inserts: (1) unc-119 promoter, unc-119 and unc-119 3'UTR cassette and (2) pie-1 promoter, MluI/BamHI cloning site and pie-1 3'UTR

Please see plasmid map link above for more details

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPK605 was a gift from Patricia Kuwabara (Addgene plasmid # 38148 ; http://n2t.net/addgene:38148 ; RRID:Addgene_38148)