|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26955||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 17037
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsccdB survival
Gene/Insert nameunc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
SpeciesC. elegans (nematode)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Second Generation pie-1 vector for expressing ORFs in the germline
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pID2.02 was a gift from Geraldine Seydoux (Addgene plasmid # 26955 ; http://n2t.net/addgene:26955 ; RRID:Addgene_26955)
For your References section:Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos. Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G. Dev Biol. 2008 Nov 1. 323(1):76-87. 10.1016/j.ydbio.2008.07.008 PubMed 18692039