pcDNA3.1his p66shc (corrected)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32574||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 His
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSHC1 (a.k.a. SHC, SHCA)
- Promoter CMV
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
This plasmid is a corrected version of pcDNA3.1his p66shc (Addgene plasmid 10972 http://www.addgene.org/10972). D2N and S60P mutations present in the original sample have been corrected in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1his p66shc (corrected) was a gift from Toren Finkel (Addgene plasmid # 32574 ; http://n2t.net/addgene:32574 ; RRID:Addgene_32574)
For your References section:Redox regulation of forkhead proteins through a p66shc-dependent signaling pathway. Nemoto S, Finkel T. Science. 2002 Mar 29. 295(5564):2450-2. 10.1126/science.1069004 PubMed 11884717