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pcDNA3.1his p66shc (corrected)
(Plasmid #32574)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32574 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p66shc
  • Species
    H. sapiens (human)
  • Entrez Gene
    SHC1 (a.k.a. SHC, SHCA)
  • Promoter CMV
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

This plasmid is a corrected version of pcDNA3.1his p66shc (Addgene plasmid 10972 http://www.addgene.org/10972). D2N and S60P mutations present in the original sample have been corrected in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1his p66shc (corrected) was a gift from Toren Finkel (Addgene plasmid # 32574 ; http://n2t.net/addgene:32574 ; RRID:Addgene_32574)
  • For your References section:

    Redox regulation of forkhead proteins through a p66shc-dependent signaling pathway. Nemoto S, Finkel T. Science. 2002 Mar 29. 295(5564):2450-2. 10.1126/science.1069004 PubMed 11884717