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pGL3-Dusp6_2KBpromoter
(Plasmid #32666)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32666 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3 (Gateway Modified)
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dusp6
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    dusp6 (a.k.a. Mkp3, wu:fd99d09)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer Luc_N_Rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Dusp6_2KBpromoter was a gift from Michael Tsang (Addgene plasmid # 32666 ; http://n2t.net/addgene:32666 ; RRID:Addgene_32666)
  • For your References section:

    Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development. Znosko WA, Yu S, Thomas K, Molina GA, Li C, Tsang W, Dawid IB, Moon AM, Tsang M. Dev Biol. 2010 Jun 1;342(1):11-25. Epub 2010 Mar 24. 10.1016/j.ydbio.2010.03.011 PubMed 20346941