-1748 human cyclin D1 promoter pGL3Basic
(Plasmid #32726)

• Depositing Lab: Frank McCormick
• Publication: Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	32726	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pGL3Basic
  (Search Vector Database)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Luciferase ; reporter gene

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CCND1 promoter
• Species: H. sapiens (human)
• Insert Size (bp): 1882
• Entrez Gene: CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
• Promoter: -1748CCND1 promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: Hind3 (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: GLprimer2

Resource Information

• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License
• Articles Citing this Plasmid:
  • 6 References