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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 32726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL3Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
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Vector typeLuciferase ; reporter gene
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCCND1 promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1882
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Entrez GeneCCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
- Promoter -1748CCND1 promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site Hind3 (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer GLprimer2 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
-1748 human cyclin D1 promoter pGL3Basic was a gift from Frank McCormick (Addgene plasmid # 32726 ; http://n2t.net/addgene:32726 ; RRID:Addgene_32726) -
For your References section:
Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Tetsu O, McCormick F. Nature. 1999 Apr 1;398(6726):422-6. 10.1038/18884 PubMed 10201372