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10-4 cyclin E promoter
(Plasmid #8458)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8458 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL-2
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human cyclin E promoter
  • Alt name
    CCNE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    X95406
  • Entrez Gene
    CCNE1 (a.k.a. CCNE, pCCNE1)

Cloning Information

Resource Information

Depositor Comments

Promoter sequence -363 to +1007; see author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    10-4 cyclin E promoter was a gift from Bob Weinberg (Addgene plasmid # 8458 ; http://n2t.net/addgene:8458 ; RRID:Addgene_8458)
  • For your References section:

    Regulation of cyclin E transcription by E2Fs and retinoblastoma protein. Geng Y, Eaton EN, Picon M, Roberts JM, Lundberg AS, Gifford A, Sardet C, Weinberg RA. Oncogene 1996 Mar 21;12(6):1173-80. PubMed 8649818