Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

10-4 cyclin E promoter
(Plasmid #8458)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8458 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL-2
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human cyclin E promoter
  • Alt name
    CCNE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    X95406
  • Entrez Gene
    CCNE1 (a.k.a. CCNE, pCCNE1)

Cloning Information

Resource Information

Depositor Comments

Promoter sequence -363 to +1007; see author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    10-4 cyclin E promoter was a gift from Bob Weinberg (Addgene plasmid # 8458 ; http://n2t.net/addgene:8458 ; RRID:Addgene_8458)
  • For your References section:

    Regulation of cyclin E transcription by E2Fs and retinoblastoma protein. Geng Y, Eaton EN, Picon M, Roberts JM, Lundberg AS, Gifford A, Sardet C, Weinberg RA. Oncogene 1996 Mar 21;12(6):1173-80. PubMed 8649818