EE-mut SV40 luc
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8460||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5789
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehuman cyclin E promoter fragment, mutant
SpeciesH. sapiens (human)
Insert Size (bp)115
MutationE2F sites I, II, and III mutated.
Entrez GeneCCNE1 (a.k.a. CCNE, pCCNE1)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer LucNRev (Common Sequencing Primers)
Promoter sequence -363 to -248 containing mutations at E2F sites I, II, III; see author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EE-mut SV40 luc was a gift from Bob Weinberg (Addgene plasmid # 8460 ; http://n2t.net/addgene:8460 ; RRID:Addgene_8460)
For your References section:Regulation of cyclin E transcription by E2Fs and retinoblastoma protein. Geng Y, Eaton EN, Picon M, Roberts JM, Lundberg AS, Gifford A, Sardet C, Weinberg RA. Oncogene 1996 Mar 21;12(6):1173-80. PubMed 8649818
Map uploaded by the depositor.