Addgene - EE-mut SV40 luc Plasmid Data

Plasmid 8460: EE-mut SV40 luc

Gene/insert name:
human cyclin E promoter fragment, mutant
Alt name:
CCNE1
Insert size:
115
Species:
H. sapiens (human)
GenBank ID:
X95406
Entrez Gene:
CCNE1 (CCNE)
Mutation:
E2F sites I, II, and III mutated.
Vector backbone:
pGL2-Promoter
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
5789
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
LucNRev List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Promoter sequence -363 to -248 containing mutations at E2F sites I, II, III; see author's map.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of cyclin E transcription by E2Fs and retinoblastoma protein. Geng et al (Oncogene 1996 Mar 21;12(6):1173-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8460" in your Materials and Methods section.