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EE-mut SV40 luc
(Plasmid #8460)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8460 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2-Promoter
  • Backbone size w/o insert (bp) 5789
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human cyclin E promoter fragment, mutant
  • Alt name
    CCNE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    115
  • Mutation
    E2F sites I, II, and III mutated.
  • GenBank ID
    X95406
  • Entrez Gene
    CCNE1 (a.k.a. CCNE, pCCNE1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Promoter sequence -363 to -248 containing mutations at E2F sites I, II, III; see author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EE-mut SV40 luc was a gift from Bob Weinberg (Addgene plasmid # 8460 ; http://n2t.net/addgene:8460 ; RRID:Addgene_8460)
  • For your References section:

    Regulation of cyclin E transcription by E2Fs and retinoblastoma protein. Geng Y, Eaton EN, Picon M, Roberts JM, Lundberg AS, Gifford A, Sardet C, Weinberg RA. Oncogene 1996 Mar 21;12(6):1173-80. PubMed 8649818