Addgene - PANK3 Plasmid Data

Plasmid 32872: PANK3

Gene/insert name:
PANK3
Alt name:
PDB: 3SMS
Alt name:
Pantothenate kinase 3
Insert size:
1089
Species:
H. sapiens (human)
Entrez Gene:
PANK3 (DKFZp686O22247, FLJ12899, MGC16863)
Fusion protein or tag:
His tag (with Thrombin cleavage)
Terminal:
N terminal on backbone
Mutation:
amino acids 10-370 only
Vector backbone:
pET28a-LIC
(Search Vector Database)
Backbone manufacturer:
Structural Genomics Consortium (Addgene plasmid 26094)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7328
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

PDB: 3SMS. http://www.thesgc.org/structures/details?pdbid=...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32872" in your Materials and Methods section.