Addgene - PANK1 Plasmid Data

Plasmid 32871: PANK1

Gene/insert name:
PANK1
Alt name:
PDB: 3SMP
Alt name:
pantothenate kinase 1 alpha
Insert size:
1101
Species:
H. sapiens (human)
Entrez Gene:
PANK1 (MGC24596, PANK)
Fusion protein or tag:
His tag (with Thrombin cleavage)
Terminal:
N terminal on backbone
Mutation:
amino acids 231-597 only
Vector backbone:
pET28a-LIC
(Search Vector Database)
Backbone manufacturer:
Structural Genomics Consortium (Addgene plasmid 26094)
Vector type:
Bacterial Expression
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

PDB: 3SMP. http://www.thesgc.org/structures/details?pdbid=...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32871" in your Materials and Methods section.

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