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pcDNA3.1(+)/Luc2=tdT
(Plasmid #32904)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32904 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Modifications to backbone
    The luc2=tdT fusion gene was created by PCR amplification of the Luc2 gene (pGL4.10, Promega, Madison, WI) and cloned by restriction digestion upstream of the tdTomato gene in the vector pRSET-B-tdTomato (kindly provided by Roger Tsien, UC San Diego). The luc2=tdT fusion gene was excised with EcoRI and inserted into the EcoRI site in the pcDNA3.1(+) multiple cloning site.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Firefly Luciferase
  • Alt name
    luc2
  • Species
    Photonis pyralis (firefly)
  • Insert Size (bp)
    3129
  • GenBank ID
    AY738222
  • Promoter CMV-IE
  • Tag / Fusion Protein
    • tdTomato red fluorescent protein (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tandem Tomato Red Fluorescent Protein
  • Species
    Discosoma
  • GenBank ID
    AY678269
  • Promoter n/a

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer pcDNA3.1/BGH reverse primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)/Luc2=tdT was a gift from Christopher Contag (Addgene plasmid # 32904 ; http://n2t.net/addgene:32904 ; RRID:Addgene_32904)
  • For your References section:

    Longitudinal, noninvasive imaging of T-cell effector function and proliferation in living subjects. Patel MR, Chang YF, Chen IY, Bachmann MH, Yan X, Contag CH, Gambhir SS. Cancer Res. 2010 Dec 15;70(24):10141-9. 10.1158/0008-5472.CAN-10-1843 PubMed 21159636