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pHIV-Luc-ZsGreen
(Plasmid #39196)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39196 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHIV-Zsgreen
  • Total vector size (bp) 9312
  • Modifications to backbone
    This plasmid was generated by cloning Luc2P (firefly luciferase) into the EcoRI and XbaI sites of pHIV-ZsGreen (Addgene Plasmid 18121).
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Firefly Luciferase Luc2P
  • Insert Size (bp)
    1600
  • Promoter EF1-alpha

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF1a Fwd Primer
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ZsGreen
  • Insert Size (bp)
    700
  • Promoter EF1-alpha

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site MscI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer pBluescriptKS Primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The backbone plasmid, HIV-Zsgreen, was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. Luciferase (Luc2P) was cloned into the EcoRI and XbaI sites to generate this bicistronic lentivirus that expresses both Luciferase and Zsgreen.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-Luc-ZsGreen was a gift from Bryan Welm (Addgene plasmid # 39196 ; http://n2t.net/addgene:39196 ; RRID:Addgene_39196)