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Plasmid 21375: pHIV-Luciferase
  • self-inactivating lentiviral plasmid for co-expression of your gene of interest and Luciferase

  • None

  • pSICO
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 8713

  • Unknown

  • 5'-TGGAATTTGCCCTTTTTGAG-3' List of Sequencing Primers

  • 5'-AGGAACTGCTTCCTTCACGA-3'

  • Ampicillin

  • Stbl3

  • 37

  • Growth in Stbl3 is preferred

  • High Copy

  • pSICO was obtained from Tyler Jacks laboratory.

  • View sequences (3)
  • Bryan Welm

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published.

NOTE: Addgene's stock is missing bp 6102-6190 of the depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21375" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only