Purpose(Empty Backbone) self-inactivating lentiviral plasmid for co-expression of your gene of interest and Luciferase
- Backbone size (bp) 8713
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsGrowth in Stbl3 is preferred
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer 5'-TGGAATTTGCCCTTTTTGAG-3'
- 3′ sequencing primer 5'-AGGAACTGCTTCCTTCACGA-3' (Common Sequencing Primers)
The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published.
NOTE: Addgene's stock is missing bp 6102-6190 of the depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHIV-Luciferase was a gift from Bryan Welm (Addgene plasmid # 21375)
Map generated by Addgene from full sequence supplied by depositor.