Addgene - pHIV-Luciferase Plasmid Data

Plasmid 21375: pHIV-Luciferase

Gene/insert name:
None
Vector backbone:
pSICO
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size (bp):
8713
5' sequencing primer:
5â€™-TGGAATTTGCCCTTTTTGAG-3â€™ List of Sequencing Primers
3' sequencing primer:
5â€™-AGGAACTGCTTCCTTCACGA-3â€™
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Growth in Stbl3 is preferred
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pSICO was obtained from Tyler Jacks laboratory.
Sequence: View sequences (2)
Principal Investigator:
Bryan Welm
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21375" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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