1
Plasmid 21375: pHIV-Luciferase
  • self-inactivating lentiviral plasmid for co-expression of your gene of interest and Luciferase

  • None

  • pSICO
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 8713

  • Unknown

  • 5'-TGGAATTTGCCCTTTTTGAG-3' List of Sequencing Primers

  • 5'-AGGAACTGCTTCCTTCACGA-3'

  • Ampicillin

  • Stbl3

  • 37

  • Growth in Stbl3 is preferred

  • High Copy

  • pSICO was obtained from Tyler Jacks laboratory.

  • View sequences (3)
  • Bryan Welm

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published.

NOTE: Addgene's stock is missing bp 6102-6190 of the depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21375" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only