pHIV-Luciferase
(Plasmid #21375)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Growth in Stbl3 is preferred
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer 5'-TGGAATTTGCCCTTTTTGAG-3'
  • 3′ sequencing primer 5'-AGGAACTGCTTCCTTCACGA-3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published.

NOTE: Addgene's stock is missing bp 6102-6190 of the depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-Luciferase was a gift from Bryan Welm (Addgene plasmid # 21375)