Addgene - pLVX-TIGHT-CYCLINF-Tet-OFF Plasmid Data

Plasmid 32977: pLVX-TIGHT-CYCLINF-Tet-OFF

Gene/insert name:
CYCLIN F
Species:
H. sapiens (human)
Entrez Gene:
CCNF (FBX1, FBXO1)
Fusion protein or tag:
mCherry
Terminal:
N terminal on insert
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pLVX-Tight-Puro
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7791
Promoter:
P-TIGHT
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
None
Site destroyed during cloning:
Unknown
5' sequencing primer:
LNCX_primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Michele Pagano
Terms and Licenses:
MTA
Clontech Limited Use Label License
Tet Systems Limited Use Label License

Comments:

Backbone vector, pLVX-Tight-Puro map is attached.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32977" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

18773: MSCV Myc T58A puro

19319: pLJM1-EGFP

19407: pTREtight2

25434: pMA2640