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pAC-luc-CLK
(Plasmid #33132)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 33132 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAc5.1/V5-His B
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    pAc-Luciferase was constructed by ligation of the PCR product containing the Renilla Luciferase gene and an artificial poly-linker sequence into the parent vector.
  • Vector type
    Insect Expression, Luciferase
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLK 3' UTR
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Clk (a.k.a. Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • Firefly Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer AC5
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pAc-Luc-clk was constructed by amplifying the genomic region containing the clk 3′ UTR plus 300 bases downstream from the annotated end of the mRNA and ligating the PCR product into pAc-Luciferase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-luc-CLK was a gift from Michael Rosbash (Addgene plasmid # 33132 ; http://n2t.net/addgene:33132 ; RRID:Addgene_33132)
  • For your References section:

    A role for microRNAs in the Drosophila circadian clock. Kadener S, Menet JS, Sugino K, Horwich MD, Weissbein U, Nawathean P, Vagin VV, Zamore PD, Nelson SB, Rosbash M. Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. 10.1101/gad.1819509 PubMed 19696147