Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||33132||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepAc5.1/V5-His B
Modifications to backbonepAc-Luciferase was constructed by ligation of the PCR product containing the Renilla Luciferase gene and an artificial poly-linker sequence into the parent vector.
Vector typeInsect Expression, Luciferase
Selectable markersHygromycin, Blasticidin
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCLK 3' UTR
SpeciesD. melanogaster (fly)
Entrez GeneClk (a.k.a. Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk)
- Promoter Actin 5C
/ Fusion Protein
- Firefly Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer AC5 (Common Sequencing Primers)
pAc-Luc-clk was constructed by amplifying the genomic region containing the clk 3′ UTR plus 300 bases downstream from the annotated end of the mRNA and ligating the PCR product into pAc-Luciferase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC-luc-CLK was a gift from Michael Rosbash (Addgene plasmid # 33132 ; http://n2t.net/addgene:33132 ; RRID:Addgene_33132)
For your References section:A role for microRNAs in the Drosophila circadian clock. Kadener S, Menet JS, Sugino K, Horwich MD, Weissbein U, Nawathean P, Vagin VV, Zamore PD, Nelson SB, Rosbash M. Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. 10.1101/gad.1819509 PubMed 19696147