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pUNos_C1
(Plasmid #33297)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pUC18 and pAHC25
  • Backbone manufacturer
    ATCC(pUC18) and Christensen and Quail(pAHC25)
  • Backbone size (bp) 4930
  • Modifications to backbone
    pUC18 was digested by HindIII and EcoRI, then DNA fragment containing maize ubiquitin promoter, E. coli uidA gene(GUS) and NOS terminator which is obtained from pAHC25 was inserted. GUS region was cut out by using SmaI and SacI, and Gateway cassette C1 which is obtained from pMDC32 was inserted there. SmaI site was destroyed by this procedure.
  • Vector type
    Plant expression
  • Promoter maize ubiquitin (Ubi-1)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Low Copy

Sequence Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUNos_C1 was a gift from Jane Glazebrook & Fumi Katagiri (Addgene plasmid # 33297)