1
Plasmid 33297: pUNos_C1
  • None

  • pUC18 and pAHC25
    (Search Vector Database)

  • ATCC(pUC18) and Christensen and Quail(pAHC25)

  • Plant expression

  • 4930

  • pUC18 was digested by HindIII and EcoRI, then DNA fragment containing maize ubiquitin promoter, E. coli uidA gene(GUS) and NOS terminator which is obtained from pAHC25 was inserted. GUS region was cut out by using SmaI and SacI, and Gateway cassette C1 which is obtained from pMDC32 was inserted there. SmaI site was destroyed by this procedure.

  • maize ubiquitin (Ubi-1)

  • Ampicillin and Chloramphenicol

  • DB3.1

  • 37

  • Low Copy

  • View sequences (2)
  • View map

  • Jane Glazebrook

  • Fumi Katagiri

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33297" in your Materials and Methods section.