Addgene - CMV500 empty vector Plasmid Data

Plasmid 33348: CMV500 empty vector

Gene/insert name:
None
Vector backbone:
Rc/CMV
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size (bp):
5500
Promoter:
CMV
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Charles Vinson
Terms and Licenses:
MTA

Comments:

The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with HindIII and XbaI, and the following annealed oligonucleotide was inserted: 5′-AGCTCCACCATGGACTACAAGGACGACGATGACAAGCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGCTTGTCATCGTCGTCCTTGTAGTCCATGGTGG-3′ (underlined bases are restriction site for XbaI). The HindIII restriction site was not regenerated and was reintroduced downstream of the FLAG sequence (DYKDDDDK).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: SREBP-1 dimerization specificity maps to both the helix-loop-helix and leucine zipper domains: use of a dominant negative. Rishi et al (J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33348" in your Materials and Methods section.