Addgene - pcDNA3.1 - HA-KLF4 FL delta K5S Plasmid Data

Plasmid 34594: pcDNA3.1 - HA-KLF4 FL delta K5S

Gene/insert name:
KLF4
Alt name:
GKLF
Alt name:
EZF
Insert size:
2300
Species:
H. sapiens (human)
GenBank ID:
AF105036
Entrez Gene:
KLF4 (RP11-150J11.1, EZF, GKLF)
Fusion protein or tag:
HA tag
Terminal:
N terminal on insert
Mutation:
deletion of K5S, corresponding to bases 1781 to 2086 of GenBank accession number AF105036, by deletion of an internal EcoRI fragment; K5S contains the miR-206 and miR-344 binding sites.
Vector backbone:
pcDNA3.1+
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5428
Promoter:
CMV
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
use KLF4 internal primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Michael Ruppert
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin et al (Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34594" in your Materials and Methods section.