|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35482||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Modifications to backboneVector also contains an IRES-Neo insert
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt nameSignal transducer and activator of transcription 6
SpeciesH. sapiens (human)
Insert Size (bp)2544
Entrez GeneSTAT6 (a.k.a. D12S1644, IL-4-STAT, STAT6B, STAT6C)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-forward (Common Sequencing Primers)
To generate pCMV-STAT6-IRES-Neo, the NotI-XbaI fragment of pCMV-Stat6 (IMAGE clone info: clone="MGC:90177 IMAGE:5530399") was replaced with that of pCMV-Fcgamma-RIIa-IRES-neo (Addgene plasmid ID 14948).
Lab ID for pCMV-STAT6-IRES-Neo: pANG194.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-STAT6-IRES-Neo was a gift from Axel Nohturfft (Addgene plasmid # 35482 ; http://n2t.net/addgene:35482 ; RRID:Addgene_35482)