p2xSTAT6-Luc2P
(Plasmid #35486)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGL4
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4267
  • Modifications to backbone
    Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] was modified by removing the hygromycin cassette as a 1782-bp XbaI fragment and by replacing the NF-κB promoter.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    STAT6 promoter
  • Promoter 2x Stat6-c/EBP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]
  • Terms and Licenses

Depositor Comments

KpnI--BglII fragment of precursor plasmid, containing an NFkappaB promoter, was replaced with a pair of annealed oligonucleotides containing two tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG185.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2xSTAT6-Luc2P was a gift from Axel Nohturfft (Addgene plasmid # 35486)