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Plasmid 35486: p2xSTAT6-Luc2P
  • STAT6 promoter

  • pGL4
    (Search Vector Database)

  • Promega

  • Mammalian Expression, Luciferase

  • 4267

  • Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] was modified by removing the hygromycin cassette as a 1782-bp XbaI fragment and by replacing the NF-κB promoter.

  • 2x Stat6-c/EBP

  • KpnI

  • No

  • BglII

  • Yes

  • RVprimer3 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]

  • View sequences (2)
  • Axel Nohturfft

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

KpnI--BglII fragment of precursor plasmid, containing an NFkappaB promoter, was replaced with a pair of annealed oligonucleotides containing two tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG185.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35486" in your Materials and Methods section.