1
Plasmid 35486: p2xSTAT6-Luc2P
  • STAT6 promoter

  • pGL4
    (Search Vector Database)

  • Promega

  • Mammalian Expression, Luciferase

  • 4267

  • Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] was modified by removing the hygromycin cassette as a 1782-bp XbaI fragment and by replacing the NF-κB promoter.

  • 2x Stat6-c/EBP

  • KpnI

  • No

  • BglII

  • Yes

  • RVprimer3 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]

  • View sequences (2)
  • Axel Nohturfft

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

KpnI--BglII fragment of precursor plasmid, containing an NFkappaB promoter, was replaced with a pair of annealed oligonucleotides containing two tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG185.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35486" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only