- Backbone size w/o insert (bp) 4267
Modifications to backbonePlasmid pGL4.32[luc2P/NF-κB-RE/Hygro] was modified by removing the hygromycin cassette as a 1782-bp XbaI fragment and by replacing the NF-κB promoter.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameSTAT6 promoter
- Promoter 2x Stat6-c/EBP
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
KpnI--BglII fragment of precursor plasmid, containing an NFkappaB promoter, was replaced with a pair of annealed oligonucleotides containing two tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG185.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2xSTAT6-Luc2P was a gift from Axel Nohturfft (Addgene plasmid # 35486)
Map generated by Addgene from full sequence supplied by depositor.