|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35554||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4267
Modifications to backbonePlasmid pGL4.32[luc2P/NF-κB-RE/Hygro] (Promega) was modified by removing the 1782-bp XbaI fragment and by replacing the NF-κB promoter.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert name4xStat6 promoter
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)156
- Promoter 4xStat6
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byNote: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]
Terms and Licenses
Articles Citing this Plasmid
This plasmid was generated by replacing the HIndIII(blunt)-AdeI fragment of p2xSTAT6-Luc2P (Addgene plasmid 35486) with the KpnI(blunt)-AdeI fragment of p2xSTAT6-Luc2P. The promoter insert consists of four tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG193.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p4xSTAT6-Luc2P was a gift from Axel Nohturfft (Addgene plasmid # 35554 ; http://n2t.net/addgene:35554 ; RRID:Addgene_35554)