Addgene - p4xSTAT6-Luc2P Plasmid Data

Plasmid 35554: p4xSTAT6-Luc2P

Gene/insert name:
4xStat6 promoter
Insert size:
156
Species:
H. sapiens (human), Synthetic
Vector backbone:
pGL4
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4267
Modifications to Backbone:
Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] (Promega) was modified by removing the 1782-bp XbaI fragment and by replacing the NF-κB promoter.
Promoter:
4xStat6
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
Yes
5' sequencing primer:
RVprimer3 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]
Sequence: View sequences (2)
Principal Investigator:
Axel Nohturfft
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

This plasmid was generated by replacing the HIndIII(blunt)-AdeI fragment of p2xSTAT6-Luc2P (Addgene plasmid 35486) with the KpnI(blunt)-AdeI fragment of p2xSTAT6-Luc2P. The promoter insert consists of four tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG193.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35554" in your Materials and Methods section.