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IgK-IFN-luc
(Plasmid #14886)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14886 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IgK-IFN
  • Alt name
    IFNB1
  • Alt name
    IgK2‐IFN‐LUC reporter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    80
  • Entrez Gene
    IFNB1 (a.k.a. IFB, IFF, IFN-beta, IFNB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contains two copies of the immunoglobulin kappa light chain kappa-B site (5'-GGGGACTTTCC-3') upstream of the interferon-beta minimal promoter (–55 to +19) (Fujita et al., 1987) driving luciferase expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IgK-IFN-luc was a gift from David Baltimore (Addgene plasmid # 14886)
  • For your References section:

    CARD11 mediates factor-specific activation of NF-kappaB by the T cell receptor complex. Pomerantz JL, Denny EM, Baltimore D. EMBO J. 2002 Oct 1. 21(19):5184-94. 10.1093/emboj/cdf505 PubMed 12356734