1
Plasmid 14886: IgK-IFN-luc
  • IgK-IFN

  • IFNB1

  • 80

  • H. sapiens (human)

  • IFNB1 (RP11-113D19.1, IFB, IFF, IFNB)

  • na
    (Search Vector Database)

  • Mammalian Expression, Luciferase

  • 5800

  • SacII

  • No

  • HindIII

  • No

  • na List of Sequencing Primers

  • LucNrev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • View map

  • David Baltimore

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

Contains two copies of the immunoglobulin kappa light chain kappa-B site (5'-GGGGACTTTCC-3') upstream of the interferon-beta minimal promoter (–55 to +19) (Fujita et al., 1987) driving luciferase expression.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: CARD11 mediates factor-specific activation of NF-kappaB by the T cell receptor complex. Pomerantz et al (EMBO J. 2002 Oct 1. 21(19):5184-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14886" in your Materials and Methods section.