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Plasmid 34596: pLJD-KLF4-ER
  • KLF4

  • GKLF

  • EZF

  • 2400

  • H. sapiens (human)

  • AF105036

  • KLF4 (RP11-150J11.1, EZF, GKLF)

  • Myc epitope tag

  • N terminal on insert

  • estrogen receptor fusion

  • C terminal on insert

  • pLJD
    (Search Vector Database)

  • LT Chow/UAB

  • Mammalian Expression, Retroviral

  • 6600

  • LTR

  • LTR

  • BamHI

  • No

  • SalI

  • No

  • pBluescriptSK List of Sequencing Primers

  • use ER internal primers

  • Kanamycin

  • XL1 Blue

  • 37

  • use Kan at 50ug/ml

  • Unknown

  • Neomycin

  • insert transferred from pBABE-puro KLF4-ER, see Oncogene 2005 Foster KW et al. PMID:15674344

  • View sequences (4)
  • View map

  • Michael Ruppert

  • UBMTA

Comments: 

see information provided for pLJD vector

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin et al (Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34596" in your Materials and Methods section.