pLJD-KLF4-ER
(Plasmid #34596)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLJD
  • Backbone manufacturer
    LT Chow/UAB
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    use Kan at 50ug/ml
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    KLF4
  • Alt name
    GKLF
  • Alt name
    EZF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • GenBank ID
    AF105036
  • Entrez Gene
    KLF4 (a.k.a. RP11-150J11.1, EZF, GKLF)
  • Promoter LTR
  • Tags / Fusion Proteins
    • Myc epitope tag (N terminal on insert)
    • estrogen receptor fusion (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBluescriptSK
  • 3′ sequencing primer use ER internal primers
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    insert transferred from pBABE-puro KLF4-ER, see Oncogene 2005 Foster KW et al. PMID:15674344
  • Terms and Licenses

Depositor Comments

see information provided for pLJD vector

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJD-KLF4-ER was a gift from Michael Ruppert (Addgene plasmid # 34596)
  • For your References section:

    A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. 10.1128/MCB.01189-10 PubMed 21518959