Addgene - pLJD-KLF4-ER Plasmid Data

Plasmid 34596: pLJD-KLF4-ER

Gene/insert name:
KLF4
Alt name:
GKLF
Alt name:
EZF
Insert size:
2400
Species:
H. sapiens (human)
GenBank ID:
AF105036
Entrez Gene:
KLF4 (RP11-150J11.1, EZF, GKLF)
Fusion protein or tag:
Myc epitope tag
Terminal:
N terminal on insert
Fusion protein or tag:
estrogen receptor fusion
Terminal:
C terminal on insert
Vector backbone:
pLJD
(Search Vector Database)
Backbone manufacturer:
LT Chow/UAB
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6600
Promoter:
LTR
Promoter:
LTR
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBluescriptSK List of Sequencing Primers
3' sequencing primer:
use ER internal primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
Growth instructions:
use Kan at 50ug/ml
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
insert transferred from pBABE-puro KLF4-ER, see Oncogene 2005 Foster KW et al. PMID:15674344
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Michael Ruppert
Terms and Licenses:
MTA

Comments:

see information provided for pLJD vector

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin et al (Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34596" in your Materials and Methods section.