RCAS(B)-Flag-PLZF
(Plasmid #34887)

• Depositing Lab: Peter Vogt
• Publication: Shi et al Int J Cancer. 2009 Oct 1;125(7):1558-65.

Backbone

• Vector backbone: RCAS(B)
• Vector type: Retroviral ; avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLZF
• Species: H. sapiens (human)
• Entrez Gene: PLZF
• Entrez Gene: ZBTB16 (a.k.a. PLZF, ZNF145)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SfiI (unknown if destroyed)
• 3′ cloning site: SfiI (unknown if destroyed)
• 5′ sequencing primer: RCAS-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A HindIII fragment containing an N-terminally Flag-tagged human PLZF cDNA in pRSV-PLZF (a kind gift from Dr. Jonathan D. Licht, Northwestern University, Chicago) was cloned into the HindIII sites of the adaptor vector pBSFI, followed by subcloning of the SfiI DNA fragment into the SfiI site of the avian retrovirus vector RCAS(B). The RCAS vector is based on RCAS.Sfi, a derivative of RCAS containing SfiI(A) and SfiI(B) sites for unidirectional cloning (Aoki et al., 1998).