Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34913||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeAAV, Cre/Lox
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namepostsynaptic mGRASP
SpeciesM. musculus (mouse)
Insert Size (bp)1722
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer gcggctctagagcctctgcta
- 3′ sequencing primer ttaaagcagcgtatccacat (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:paavCAG-Jx-rev-post-mGRASP-2A-dTomato was a gift from Jinhyun Kim (Addgene plasmid # 34913 ; http://n2t.net/addgene:34913 ; RRID:Addgene_34913)
For your References section:mGRASP enables mapping mammalian synaptic connectivity with light microscopy. Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC. Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784. 10.1038/nmeth.1784 PubMed 22138823
Map uploaded by the depositor.