Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

pBbA5c-MevT-MBIS
(Plasmid #35150)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35150 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
  • Species
    S. cerevisiae (budding yeast); E. Coli
  • Promoter LacUV5,

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1, BglII (not destroyed)
  • 3′ cloning site BamH1, Xho1 (unknown if destroyed)
  • 5′ sequencing primer gatcgtttaggcacccca
  • 3′ sequencing primer CGTTTTATTTGATGCCTGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5c-MevT-MBIS was a gift from Jay Keasling & Taek Soon Lee (Addgene plasmid # 35150)
  • For your References section:

    Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. 10.1038/ncomms1494 PubMed 21952217