pcDNA5FRT PUR mGSK3A K148A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35158||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5100
Modifications to backboneA mammalian expression plasmid containing a single FRT recombination site was created by splicing the CMV promoter, multiple cloning site, polyA signal, and puromycin-resistance cassette from pPUR (Clontech) into the BspEI and Bst11071 sites of pcDNA5/FRT (Invitrogen) to yield pcDNA5/FRT_puro.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationK148A (dominant negative)
Entrez GeneGsk3a (a.k.a. 2700086H06Rik)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
S21A mutation shown on author's map indicates where the S21 is, for scientists that wish to mutate it. This plasmid carries a serine (not alanine) at amino acid position 21.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA5FRT PUR mGSK3A K148A was a gift from Jim Woodgett (Addgene plasmid # 35158 ; http://n2t.net/addgene:35158 ; RRID:Addgene_35158)
For your References section:Functional redundancy of GSK-3alpha and GSK-3beta in Wnt/beta-catenin signaling shown by using an allelic series of embryonic stem cell lines. Doble BW, Patel S, Wood GA, Kockeritz LK, Woodgett JR. Dev Cell. 2007 Jun;12(6):957-71. 10.1016/j.devcel.2007.04.001 PubMed 17543867
Map uploaded by the depositor.