pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35191||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3956
Vector typeBacterial Expression, Cre/Lox
Growth in Bacteria
Gene/Insert nameloxP KAN loxP 24xPP7SL MDN1
SpeciesS. cerevisiae (budding yeast)
- Cloning method TOPO Cloning
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.
Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.
Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDZ207 (loxP KAN loxP 24xPP7SL MDN1) was a gift from Robert Singer (Addgene plasmid # 35191)
For your References section:Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033