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Plasmid 35191: pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
  • loxP KAN loxP 24xPP7SL MDN1

  • S. cerevisiae (budding yeast)

  • pCR4
    (Search Vector Database)

  • Invitrogen

  • Bacterial Expression, Cre/Lox

  • 3956

  • TOPO Cloning

  • T3 List of Sequencing Primers

  • T7

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • View sequences (2)
  • Robert Singer

  • MTA

Comments: 

This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.

Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.

Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson et al (Science. 2011 Apr 22;332(6028):475-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35191" in your Materials and Methods section.