Addgene - pDZ207 (loxP KAN loxP 24xPP7SL MDN1) Plasmid Data

Plasmid 35191: pDZ207 (loxP KAN loxP 24xPP7SL MDN1)

Gene/insert name:
loxP KAN loxP 24xPP7SL MDN1
Species:
S. cerevisiae (budding yeast)
Vector backbone:
pCR4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Bacterial Expression, Cre/Lox
Backbone size w/o insert (bp):
3956
Cloning method:
TOPO Cloning
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Robert Singer
Terms and Licenses:
MTA

Comments:

This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.

Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.

Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson et al (Science. 2011 Apr 22;332(6028):475-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35191" in your Materials and Methods section.