pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
(Plasmid #35191)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCR4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3956
  • Vector type
    Bacterial Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    loxP KAN loxP 24xPP7SL MDN1
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.

Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.

Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDZ207 (loxP KAN loxP 24xPP7SL MDN1) was a gift from Robert Singer (Addgene plasmid # 35191)
  • For your References section:

    Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033