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pDZ276 (pURA MET28 PCP-2x-yeGFP)
(Plasmid #35194)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35194 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PCP-2x-yeGFP
  • Alt name
    2X yeast enhanced green fluorescent protein
  • Species
    Synthetic

Resource Information

Depositor Comments

This plasmid expresses a chimeric RNA-binding protein fused to GFP used to detect mRNAs that contain multiple copies of an RNA sequence (see Addgene plasmid #'s 35191, 35192 & 35196). See associated publication for detailed methods and use of this plasmid.

Please note that Addgene's sequencing results identified discrepancies when compared to the assembled sequence provided by the depositing laboratory. The first GFP construct in this plasmid (bp#441-1154) is yeast codon optimized and contains a E124K mutation and amino acid 206 is A (not K, as suggested by the depositor's sequence). The second GFP construct in this plasmid (bp# 1212-1928) is NOT yeast codon optimized.

This construct has successfully been used by the depositing laboratory as detailed in the associated publication and the sequencing discrepancies are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDZ276 (pURA MET28 PCP-2x-yeGFP) was a gift from Robert Singer (Addgene plasmid # 35194 ; http://n2t.net/addgene:35194 ; RRID:Addgene_35194)
  • For your References section:

    Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033