Addgene - TOP-GFP Plasmid Data

Plasmid 35489: TOP-GFP

Gene/insert name:
7xTOP-GFP
Vector backbone:
pRRLSIN.cPPT.PGK-GFP.WPRE
(Search Vector Database)
Backbone manufacturer:
Didier Trono
Vector type:
Mammalian Expression, Lentiviral
Promoter:
7xTCF/LEF
Cloning site 5':
EcoRV
Site destroyed during cloning:
No
Cloning site 3':
BamH1
Site destroyed during cloning:
No
5' sequencing primer:
CGTCGCCGTCCAGCTCGACCAG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
This plasmid is very low copy and may require longer incubation times
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
We inserted the 7xTCF/LEF promoter cassette from the M50 Super-TOP flash plasmid (Randall Moon) into the pRRLSIN.cPPT.PGK-GFP.WPRE vector (Didier Trono), replacing the PGK promoter.
Sequence: View sequences (2)
Principal Investigator:
Ramesh Shivdasani
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Differential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling. Horst et al (Cancer Res. 2012 Feb 8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35489" in your Materials and Methods section.