Addgene - pLenti-CaMKIIa-eArchT 3.0-EYFP Plasmid Data

Plasmid 35513: pLenti-CaMKIIa-eArchT 3.0-EYFP

Gene/insert name:
eArchT 3.0-EYFP
Insert size:
1548
Species:
Halorubrum sp. TP009
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
Enhanced trafficking signal and ER export signal added
Vector backbone:
pLenti
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9245
Modifications to Backbone:
Addition of a CaMKIIa promoter and a WPRE
Promoter:
CaMKIIa
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
AGTCCTGCAGTATTGTGTAT List of Sequencing Primers
3' sequencing primer:
GCAATAGCATGATACAAAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
ArchT as reported by the Boyden Lab was synthesized by DNA 2.0 and fused with EYFP
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
MTA

Comments:

The enhanced trafficking signals allow for better membrane targeting in mammalian cells resulting in 3-5 fold increase in currents.

Please note that there may be several sequence discrepancies between depositor's reference sequence and Addgene's quality control sequence. These changes are in vector regions only and should not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. Mattis et al (Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35513" in your Materials and Methods section.