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Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.
Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
35505pAAV-CaMKIIa-hChR2(E123A)-EYFPAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.
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35506pAAV-CaMKIIa-hChR2(E123A)-mCherryAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.
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35507pAAV-Ef1a-DIO hChR2(E123A)-EYFPAAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.
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35508pAAV-Ef1a-DIO hChR2(E123A)-mCherryAAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.
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35509pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFPAAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
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35510pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherryAAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.
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35511pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFPAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.
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35512pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherryAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.
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35513pLenti-CaMKIIa-eArchT 3.0-EYFPLentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.
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35514pLenti-CaMKIIa-eArch 3.0-EYFPLentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.
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35515pLenti-CaMKIIa-Mac 3.0-EYFPLentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.
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35516pAAV-CaMKIIa-eArch 3.0-EYFPAAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.
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