Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.
Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 35505 | pAAV-CaMKIIa-hChR2(E123A)-EYFP | AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control. |
| 35506 | pAAV-CaMKIIa-hChR2(E123A)-mCherry | AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control. |
| 35507 | pAAV-Ef1a-DIO hChR2(E123A)-EYFP | AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control. |
| 35508 | pAAV-Ef1a-DIO hChR2(E123A)-mCherry | AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control. |
| 35509 | pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP | AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. |
| 35510 | pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry | AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control. |
| 35511 | pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP | AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control. |
| 35512 | pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry | AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control. |
| 35513 | pLenti-CaMKIIa-eArchT 3.0-EYFP | Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition. |
| 35514 | pLenti-CaMKIIa-eArch 3.0-EYFP | Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition. |
| 35515 | pLenti-CaMKIIa-Mac 3.0-EYFP | Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition. |
| 35516 | pAAV-CaMKIIa-eArch 3.0-EYFP | AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition. |