Addgene - pLenti-PGK-KRAS4A(G12V) Plasmid Data

Plasmid 35634: pLenti-PGK-KRAS4A(G12V)

Gene/insert name:
K-Ras4A(G12V)
Alt name:
KRAS4A
Insert size:
550
Species:
H. sapiens (human)
Entrez Gene:
KRAS (C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2)
Fusion protein or tag:
V5
Terminal:
N terminal on backbone
Mutation:
Glycine 12 to Valine
Vector backbone:
pLenti-PGK-Hygro
(Search Vector Database)
Backbone manufacturer:
Eric Campeau
Vector type:
Lentiviral
Backbone size w/o insert (bp):
10000
Promoter:
PGK
Cloning method:
Gateway Cloning
5' sequencing primer:
attlL1 List of Sequencing Primers
3' sequencing primer:
attL2
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Principal Investigator:
Daniel Haber
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers. Singh et al (Cell. 2012 Feb 17;148(4):639-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35634" in your Materials and Methods section.