|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35633||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerEric Campeau
- Backbone size w/o insert (bp) 10000
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)550
MutationGlycine 12 to Valine
Entrez GeneKRAS (a.k.a. C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2, c-Ki-ras2)
- Promoter PGK
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer attL1
- 3′ sequencing primer attL2 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-PGK-KRAS4B(G12V) was a gift from Daniel Haber (Addgene plasmid # 35633)
For your References section:TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers. Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA, Settleman J. Cell. 2012 Feb 17;148(4):639-50. 10.1016/j.cell.2011.12.033 PubMed 22341439
Generated by Addgene from full sequence.