|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35672||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name3 miR-122 target sites
Insert Size (bp)69
- Promoter HSV-TK
- Cloning method Restriction Enzyme
- 5′ cloning site PmeI (destroyed during cloning)
- 3′ cloning site PmeI (destroyed during cloning)
- 5′ sequencing primer cccggaagaaatatatttgca (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psiCHECK-2 3xmiR-122T was a gift from Phillip Zamore (Addgene plasmid # 35672 ; http://n2t.net/addgene:35672 ; RRID:Addgene_35672)