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pEGFP.N1-HDAC6.delta Buz
(Plasmid #36190)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    HDAC6..delta Buz
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of the C-terminal ub binding domain (BUZ finger): aa1044-1215
  • Entrez Gene
    HDAC6 (a.k.a. JM21, CPBHM, HD6, PPP1R90)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP.N1-HDAC6.delta Buz was a gift from Tso-Pang Yao (Addgene plasmid # 36190)
  • For your References section:

    The microtubule-associated histone deacetylase 6 (HDAC6) regulates epidermal growth factor receptor (EGFR) endocytic trafficking and degradation. Gao YS, Hubbert CC, Yao TP. J Biol Chem. 2010 Apr 9. 285(15):11219-26. 10.1074/jbc.M109.042754 PubMed 20133936