Addgene - pEGFP.N1-HDAC6.delta Buz Plasmid Data

Plasmid 36190: pEGFP.N1-HDAC6.delta Buz

Gene/insert name:
HDAC6..delta Buz
Species:
H. sapiens (human)
Entrez Gene:
HDAC6 (JM21, HD6)
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Mutation:
Deletion of the C-terminal ub binding domain (BUZ finger): aa1044-1215
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Promoter:
CMV
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
None
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-Fwd List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Tso-Pang Yao
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The microtubule-associated histone deacetylase 6 (HDAC6) regulates epidermal growth factor receptor (EGFR) endocytic trafficking and degradation. Gao et al (J Biol Chem. 2010 Apr 9. 285(15):11219-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36190" in your Materials and Methods section.