Addgene - pRRL WS1.6 GFP Plasmid Data

Plasmid 36249: pRRL WS1.6 GFP

Gene/insert name:
GFP
Alt name:
EGFP
Alt name:
enhanced green fluorescent protein
Insert size:
720
Entrez Gene:
eGFP (pPRS3a_01)
Vector backbone:
pRRLSIN.cppt.PGK-GFP.WPRE Addgene 12252
(Search Vector Database)
Backbone manufacturer:
Didier Trono
Vector type:
Lentiviral
Backbone size w/o insert (bp):
6857
Modifications to Backbone:
PGK GFP removed (EcoRV and BamHI)and replaced with a multiple cloning site for addition of promoter CDS cassettes
Promoter:
WAS (human 1.6 kb)
Cloning site 5':
MluI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
gatcacgagactagcctcgag List of Sequencing Primers
3' sequencing primer:
caacgggccacaactcctc
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
Backbone is from Addgene plasmid 12252 (Didier Trono)
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
David Rawlings
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ubiquitous high-level gene expression in hematopoietic lineages provides effective lentiviral gene therapy of murine Wiskott-Aldrich Syndrome. Astrakhan et al (Blood. 2012 Mar 19. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36249" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

36247: pRRL MND GFP

36248: pRRL MND WASp

36250: pRRL WS1.6 WASp