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pBAD33.1
(Plasmid #36267)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36267 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD33
  • Modifications to backbone
    Replaced pBAD33 nucleotides between XbaI to HindIII with those of pET21b including ribosomal binding site
  • Vector type
    Bacterial Expression
  • Promoter PBAD

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Cloning Information

  • 5′ sequencing primer pBAD-F (5'-ATGCCATAGCATTTTTATCC-3')
  • 3′ sequencing primer pBAD-R (5'-GATTTAATCTGTATCAGG-3')
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD33.1 was a gift from Christian Raetz (Addgene plasmid # 36267 ; http://n2t.net/addgene:36267 ; RRID:Addgene_36267)
  • For your References section:

    Interchangeable domains in the Kdo transferases of Escherichia coli and Haemophilus influenzae. Chung HS, Raetz CR. Biochemistry. 2010 May 18;49(19):4126-37. 10.1021/bi100343e PubMed 20394418