Addgene - pEGFP-C1-hAtg2A Plasmid Data

Plasmid 36456: pEGFP-C1-hAtg2A

Gene/insert name:
autophagy-related protein 2 homolog A
Alt name:
Atg2A
Insert size:
5817
Species:
H. sapiens (human)
GenBank ID:
NM_015104
Entrez Gene:
ATG2A ()
Fusion protein or tag:
EGFP
Terminal:
N terminal on insert
Mutation:
changed Proline 656 to Arginine
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4731
Promoter:
CMV
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CGACCACTACCAGCAGAACA List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Noboru Mizushima
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Velikkakath et al (Mol Biol Cell. 2012 Mar;23(5):896-909. Epub 2012 Jan 4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36456" in your Materials and Methods section.