|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36954||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid #36953
- Backbone size w/o insert (bp) 7037
- Total vector size (bp) 10074
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Alt namePKR-like ER kinase
SpeciesH. sapiens (human)
Mutationdeletion of aa592-1061 to create dominant negative
Entrez GeneEIF2AK3 (a.k.a. PEK, PERK, WRS)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer pLXSN_5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Sugden lab plasmid p3236
dnPERK from Dr Ron Wek, Indiana University School of Medicine, Indianapolis.
When compared to the GenBank reference sequence, the full plasmid sequence indicates that the PERK insert contains the following polymorphisms: a deletion of amino acid L21 and Q166R and N490H mutations.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMMP-dnPERK-IRES-eGFP was a gift from Bill Sugden (Addgene plasmid # 36954 ; http://n2t.net/addgene:36954 ; RRID:Addgene_36954)
For your References section:The LMP1 oncogene of EBV activates PERK and the unfolded protein response to drive its own synthesis. Lee DY, Sugden B. Blood. 2008 Feb 15;111(4):2280-9. Epub 2007 Nov 27. 10.1182/blood-2007-07-100032 PubMed 18042799