Addgene - hIRE1a K599A Plasmid Data

Plasmid 20745: hIRE1a K599A

Gene/insert name:
IRE1
Insert size:
2934
Species:
H. sapiens (human)
Entrez Gene:
ERN1 (IRE1, IRE1P, IRE1a, hIRE1p, FLJ30999, MGC163277, MGC163279)
Mutation:
K599A, dominant negative kinase mutant
Vector backbone:
pLenti CMV/TO Puro DEST (670-1)
(Search Vector Database)
Backbone manufacturer:
Eric Campeau (Addgene plasmid 17293)
Vector type:
Mammalian Expression, Lentiviral ; Destination vector
Backbone size w/o insert (bp):
9594
Cloning method:
Gateway Cloning
Cloning site 5':
Gateway
Site destroyed during cloning:
Unknown
Cloning site 3':
Gateway
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
MSCV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (4)
Principal Investigator:
Fumihiko Urano
Terms and Licenses:
MTA

Comments:

IRE1a cDNA was cut out of pENTR1A with EcoRI and XhoI and ligated into pLenti CMV/TO Puro DEST through Gateway cloning.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The role of IRE1alpha in the degradation of insulin mRNA in pancreatic beta-cells. Lipson et al (PLoS ONE. 2008 . 3(2):e1648. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20745" in your Materials and Methods section.