Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA
(Plasmid #37082)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37082 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA
  • Backbone manufacturer
    K. Deisseroth Lab
  • Backbone size w/o insert (bp) 5613
  • Total vector size (bp) 7260
  • Vector type
    AAV, Cre/Lox ; Cre-Off

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hChr2(H134R)-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1647
  • Promoter EF1a
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc1 (unknown if destroyed)
  • 3′ cloning site Nhe1 (unknown if destroyed)
  • 5′ sequencing primer CTTCCATTTCAGGTGTCGTG
  • 3′ sequencing primer GCAGCGTATCCACATAGCG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Karl Deisseroth Lab, Stanford Univ.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA was a gift from Bernardo Sabatini (Addgene plasmid # 37082 ; http://n2t.net/addgene:37082 ; RRID:Addgene_37082)
  • For your References section:

    Recurrent network activity drives striatal synaptogenesis. Kozorovitskiy Y, Saunders A, Johnson CA, Lowell BB, Sabatini BL. Nature. 2012 May 13;485(7400):646-50. doi: 10.1038/nature11052. 10.1038/nature11052 PubMed 22660328