Addgene - pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA Plasmid Data

Plasmid 37082: pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

Gene/insert name:
hChr2(H134R)-mCherry
Insert size:
1647
Species:
Synthetic
Fusion protein or tag:
mCherry
Terminal:
C terminal on insert
Vector backbone:
pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA
(Search Vector Database)
Backbone manufacturer:
K. Deisseroth Lab
Vector type:
rAAV
Backbone size w/o insert (bp):
5613
Promoter:
EF1a
Cloning site 5':
Asc1
Site destroyed during cloning:
Unknown
Cloning site 3':
Nhe1
Site destroyed during cloning:
Unknown
5' sequencing primer:
CTTCCATTTCAGGTGTCGTG List of Sequencing Primers
3' sequencing primer:
GCAGCGTATCCACATAGCG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
Karl Deisseroth Lab, Stanford Univ.
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Bernardo Sabatini
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Recurrent network activity drives striatal synaptogenesis. Kozorovitskiy et al (Nature. 2012 May 13;485(7400):646-50. doi: 10.1038/nature11052. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37082" in your Materials and Methods section.