Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37382||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeInsect Expression
Growth in Bacteria
SpeciesD. melanogaster (fly)
Entrez Genehh (a.k.a. Dmel_CG4637, CG4637, Dmel\CG4637, HH, Hg, Hh, Mir, Mrt, anon-WO0134654.19, anon-WO0182946.19, bar, bar-3, bar3, hg, l(3)hh, l(3)neo56, l(3)neo57)
- Promoter actin5C
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer AC5
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
Perrimon Lab plasmid #440
pAct5C-Hh construct was generated by PCR of full-length hh from a cDNA (gift from T. Kornberg; UCSF) with a 5' BamHI site and a 3' stop followed by a NotI site
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAct5C-Hh was a gift from Norbert Perrimon (Addgene plasmid # 37382 ; http://n2t.net/addgene:37382 ; RRID:Addgene_37382)
For your References section:A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N. Nat Genet. 2005 Dec;37(12):1323-32. Epub 2005 Nov 20. 10.1038/ng1682 PubMed 16311596