pAct5C-Hh
(Plasmid #37382)

• Depositing Lab: Norbert Perrimon
• Publication: Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub 2005 Nov 20.

Backbone

• Vector backbone: pAct5C
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hedgehog
• Alt name: hh
• Species: D. melanogaster (fly)
• Entrez Gene: hh (a.k.a. Dmel_CG4637, CG4637, Dmel\CG4637, Dmhh, HH, Hg, Hh, Mir, Mrt, anon-WO0134654.19, anon-WO0182946.19, bar, bar-3, bar3, hg, l(3)hh, l(3)neo56, l(3)neo57)
• Promoter: actin5C

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: AC5
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Perrimon Lab plasmid #440

pAct5C-Hh construct was generated by PCR of full-length hh from a cDNA (gift from T. Kornberg; UCSF) with a 5' BamHI site and a 3' stop followed by a NotI site