Addgene - pLentiN 6bE6 Plasmid Data

Plasmid 37447: pLentiN 6bE6

Gene/insert name:
HPV 6bE6
Alt name:
E6
Alt name:
HPV6bgp1
Insert size:
530
Species:
HPV
GenBank ID:
NC_001355 NP_040296.1
Entrez Gene:
E6 (HPV6bgp1)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pLenti 6.3/V5 DEST
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9387
Promoter:
CMV
5' sequencing primer:
CMV forward List of Sequencing Primers
3' sequencing primer:
V5 reverse primer
Promoter:
CMV
Cloning method:
Gateway Cloning
5' sequencing primer:
CMV forward List of Sequencing Primers
3' sequencing primer:
V5 reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (1)
Principal Investigator:
Karl Munger
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Activation of cap dependent translation by mucosal Human Papillomavirus E6 proteins is dependent on the integrity of the LXXLL binding motif. Spangle et al (J Virol. 2012 May 2. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37447" in your Materials and Methods section.

Price: $65.00

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