Addgene - ss-cfSGFP2 Plasmid Data

Plasmid 37535: ss-cfSGFP2

Gene/insert name:
ss-cfSGFP2
Alt name:
cfSGFP2 with a signal sequence of a1-antitrypsin
Insert size:
873
Species:
H. sapiens (human)
GenBank ID:
NM_000295
Fusion protein or tag:
Amino
Terminal:
N terminal on insert
Mutation:
deleted amino acids 35-432
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clonetech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4105
Promoter:
CMV
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
GCAGAGCTGGTTTAGTGAACCGTCAG List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
XL10 Gold
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Ikuo Wada
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Development of cysteine-free fluorescent proteins for the oxidative environment. Suzuki et al (PLoS One. 2012;7(5):e37551. Epub 2012 May 23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37535" in your Materials and Methods section.